anonymous
  • anonymous
Can we culture neurons?
Genomes & Diversity - NYU Open Education
jamiebookeater
  • jamiebookeater
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Owlfred
  • Owlfred
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anonymous
  • anonymous
I think the ans is no.......(not sure) these cells once matured won't divide. Each type of cell has its own special requirements in terms of exact pH, nutrients, and growth factors. And, nerve cells require such specific requirements that there is no one sure way to culture them. The general process is to isolate the tissue through surgery, and then use mechanical plus enzymatic disassociation of the tissue, light centrifugation, and rinsing in media to remove enzymes then initial plating. This is actually very difficult to do in practice. Another obstacle is that primary nerve cultures (taken directly from living tissue) have a relatively short lifespan. Nerve cells are not like muscle and skin cells in that they generally lack the ability to replicate. FROM the site mentioned below Read more: http://wiki.answers.com/Q/How_does_one_culture_nerve_cells#ixzz1O0OEMcDm Nerve cell culture medias are availabe.....for growing certain viruses like RABIES........
anonymous
  • anonymous
but can we geneically modify it to induce division?

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anonymous
  • anonymous
Scientists have been able to grow neurons from human skin cells. These cells "were able to generate action potentials and many matured to receive synaptic contacts when co-cultured with primary mouse cortical neurons" I don't beleive that we can actualy implent these neurons in a human body, but that's a first step. Nature article's abstract on the subject: http://www.nature.com/nature/journal/vnfv/ncurrent/full/nature10202.html
anonymous
  • anonymous
http://4mind4life.com/blog/2008/08/18/7-scientifically-proven-ways-to-stimulate-brain-cell-growth-neurogenesis/
anonymous
  • anonymous
It has been demonstrated that neurogenesis can sometimes occur in the adult vertebrate brain, a finding that led to controversy in 1999. However, more recent studies of the age of human neurons suggest that this process occurs only for a minority of cells, and the overwhelming majority of neurons comprising the neocortex were formed before birth and persist without replacement. It is often possible for peripheral axons to regrow if they are severed. This regrowth can take a long time: after a nerve injury to the human arm, for example, it may take months for feeling to return to the hands and fingers.[citation needed] A report in Nature suggested that researchers had found a way to transform human skin cells into working nerve cells using a process called transdifferentiation in which "cells are forced to adopt new identities.
anonymous
  • anonymous
In my lab, I have been growing neurons from cerebellum tissues. The hardest part is separating them, because allego-dendrocytes turn up more.

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