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Cell Cycle Lab
• Always handle microscopes and glass slides carefully.
• Wash your hands after handling the prepared specimens.
o Compound light microscope
o Glass microscope slide with prepared onion root tip specimen
• understand and identify the stages of the cell cycle and mitosis.
• apply an analytical technique to estimate the relative length of each stage of the cell cycle.
Data and Observations:
Create a data table containing a tally of the number of cells observed in each of the following stages:
Stage Number of Cells in Part 1 Number of Cells in Part 2
Interphase 12 16
Prophase 11 10
Metaphase 4 7
Anaphase 10 16
Telophase 10 21
Cytokinesis 3 6
Record any observations about the cells you observed.
Calculate the percentage of the cell cycle spent in each stage.
Number of cells in given stage ÷ total number of cells counted × 100 = % of the cell cycle spent in this stage
Create a graph that represents the time spent in each stage of the cell cycle.
Stage Time Spent in the 1st stage Time Spent in the 2nd stage
Interphase 42.85% 57.14%
Prophase 190.90% 210%
Metaphase 275% 157.14%
Anaphase 260% 162.5%
Telophase 310% 147.61%
Cytokinesis 300% 150%
Be sure to answer the following reflection questions in the conclusion of your lab report:
1. Based on your data, what can you infer about the length of time spent in each stage of the cell cycle?