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  • 4 years ago

Explain how scientists use recombinant DNA technology to produce the drug insulin. Be sure to also explain why a gene from one organism can be expressed by another organism.

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  1. anonymous
    • 4 years ago
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  2. anonymous
    • 4 years ago
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    Second question first: you can express the gene of one organism in another one because the translation from DNA to protein is the same in all organisms. For the same nucleotide sequence, you will get the same amino acid sequence in your protein. There are some epigenetic differences between eukaryotes and prokaryotes (this means, the DNA is modified differently in the organisms). But you can avoid that. Now, to your question: We use the bacterium e.coli to synthesize recombinant insulin. Recombinant means: the protein has additional "tags" that help us purify it. Now, we CUT out the DNA sequence of insulin from human DNA and INSERT it into a gene ferry that controls the expression of the protein. This gene ferry is a circular strand of DNA, initially made from viral DNA, that now serves us. We put the gene ferry into the e.coli (process known as transfection) and wait for it to synthesize all the protein. We then destroy the bacteria and get a protein solution. We then wash everything else away and yeehaw, there is our protein. :-) (It's actually not that simple, but this is the most relevant information, I suppose. If you have any questions, feel free to ask)

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