Can somebody explain to me what Restriction Fragment Length Polymorphism is? (RFLP)
Stacey Warren - Expert brainly.com
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Ok, I did a google search. http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/bio20.swf::Restriction%20Fragment%20Length%20Polymorphisms
You start with a bunch of homologous DNA molecules. You treat them with a restriction enzyme which "digests" the DNA molecules by recognizing short sequences in the DNA and cleaving the backbones at those sites. You then run the fragments on a gel to separate them by size and transfer them to a membrane by southern blotting. Then you treat the membrane with a probe sensitive for DNA fragments of different lengths and see what binds. A RFLP occurs when the length of a detected fragment varies among individuals.
This was a long time ago for me.
But I still don't know what it is.
I understood. Thank you!
It was a lot more important before the gilded age of high throughput sequencing.