A community for students.

Here's the question you clicked on:

55 members online
  • 0 replying
  • 0 viewing

MSMR

  • 4 years ago

Can somebody explain to me what Restriction Fragment Length Polymorphism is? (RFLP)

  • This Question is Closed
  1. Preetha
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 0

    I am waiting for Blues to respond.

  2. blues
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 1

    And I'm waiting for Preetha.

  3. Preetha
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 0

    Not fair. You know this better than me, I think.

  4. Preetha
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 0

    Or MSMR

  5. MSMR
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 2

    true that.

  6. blues
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 1

    You start with a bunch of homologous DNA molecules. You treat them with a restriction enzyme which "digests" the DNA molecules by recognizing short sequences in the DNA and cleaving the backbones at those sites. You then run the fragments on a gel to separate them by size and transfer them to a membrane by southern blotting. Then you treat the membrane with a probe sensitive for DNA fragments of different lengths and see what binds. A RFLP occurs when the length of a detected fragment varies among individuals. This was a long time ago for me.

  7. Preetha
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 0

    But I still don't know what it is.

  8. MSMR
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 2

    I understood. Thank you!

  9. blues
    • 4 years ago
    Best Response
    You've already chosen the best response.
    Medals 1

    It was a lot more important before the gilded age of high throughput sequencing.

  10. Not the answer you are looking for?
    Search for more explanations.

    • Attachments:

Ask your own question

Sign Up
Find more explanations on OpenStudy
Privacy Policy

Your question is ready. Sign up for free to start getting answers.

spraguer (Moderator)
5 → View Detailed Profile

is replying to Can someone tell me what button the professor is hitting...

23

  • Teamwork 19 Teammate
  • Problem Solving 19 Hero
  • You have blocked this person.
  • ✔ You're a fan Checking fan status...

Thanks for being so helpful in mathematics. If you are getting quality help, make sure you spread the word about OpenStudy.

This is the testimonial you wrote.
You haven't written a testimonial for Owlfred.