It is known that the simple Ames test sometimes does not show known mutagens to be mutagenic.. what are the possible reasons for this? and how to counter them?

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It is known that the simple Ames test sometimes does not show known mutagens to be mutagenic.. what are the possible reasons for this? and how to counter them?

Biology
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Maybe because the Salmonella is prokaryotic cell and not a model to simulate the mutagenic effect on complex human cells.

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