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This is my scenario The heart that was found has been sitting in preservatives for almost 50 years so the DNA is most likely degraded. The heart is said to have belonged to a man who's identity has been revealed by his supposed murder. In order to see if this particular heart is the one that belonged to this man, you are going to compare the DNA found in the heart to the DNA of this man's grandson to see if they are related and the prisoner's story is true. Should I use RFLP, PCR, STR or Y chromosome analyisis?
RFLP requires a large amount of undegraded DNA, and it's hard to discern between individual genes thereby NOT making it useful for testing to see if two DNA samples are from related people. And PCR is good for small samples of DNA and degraded DNA (for example old DNA samples). However, it's very discriminating so that means it is hard to see small differences in DNA samples that you might want to use to see if idividuals are related. STR testing is really good for discriminating between 2 unrelated individuals. Or Y-Chromosome analysis because only males have the Y sex-linked chromosome, this test is used to see if DNA matches up between 2 related males
There isn't a wrong answer just a better answer. What's your thoughts?