At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.
This is my scenario The heart that was found has been sitting in preservatives for almost 50 years so the DNA is most likely degraded. The heart is said to have belonged to a man who's identity has been revealed by his supposed murder. In order to see if this particular heart is the one that belonged to this man, you are going to compare the DNA found in the heart to the DNA of this man's grandson to see if they are related and the prisoner's story is true. Should I use RFLP, PCR, STR or Y chromosome analyisis?
RFLP requires a large amount of undegraded DNA, and it's hard to discern between individual genes thereby NOT making it useful for testing to see if two DNA samples are from related people. And PCR is good for small samples of DNA and degraded DNA (for example old DNA samples). However, it's very discriminating so that means it is hard to see small differences in DNA samples that you might want to use to see if idividuals are related. STR testing is really good for discriminating between 2 unrelated individuals. Or Y-Chromosome analysis because only males have the Y sex-linked chromosome, this test is used to see if DNA matches up between 2 related males
There isn't a wrong answer just a better answer. What's your thoughts?
@bngaure, you'll make a good detective. I seriously don't understand a word of what you've typed.
Well given how you've described each option, doesn't Y chromosome analysis sound like the best choice?
i agree @InYourHead but you have to consider the dna is degraded so I would go with PCR because either way youre going to find out whether or not their related(: That was my first thought though(:
@pratu043 haha thanks im not going into that though im just taking a forensics class and I was curious to see others opinions(: