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thnx my dear fr ur corporation but wht i need is a detail of trp operon?
The genes for the five enzymes in the Trp synthesis pathway are clustered on the same chromosome in what is called the Trp operon. The Trp operon has three components: Five Structural Genes: These genes contain the genetic code for the five enzymes in the Trp synthesis pathway One Promoter: DNA segment where RNA polymerase binds and starts transcription One Operator: DNA segment found between the promoter and structural genes. It determines if transcription will take place. If the operator is turned "on", transcription will occur. When nothing is bonded to the operator, the operon is "on". RNA polymerase binds to the promoter and transcription is initiated. The five structural genes are transcribed to one mRNA strand. The mRNA will then be translated into the enzymes that control the Trp synthesis pathway. The operon is turned "off" by a specific protein called the repressor. The repressor is a product of the regulator gene which is found some distance from the operon. Transcription of the regulator produces mRNA which is translated into the repressor. The repressor is inactive in this form and can not bind properly to the operator with this conformation. To become active and bind properly to he operator, a co-repressor must associate with the repressor. The co--repressor for this system is Trp This makes sense because E. coli does not want to synthesize Trp if it is available from the environment The more Trp available, the more that can associate with repressor molecules. An active repressor binds to the operator blocking the attachment of RNA polymerase to the promoter. Without RNA polymerase, transcription and translation of the structural genes can't occur and the enzymes needed for Trp synthesis are not made. Hope this helps.