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EmmaH

What is a restriction map?

  • one year ago
  • one year ago

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  1. tammy16
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    A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes

    • one year ago
  2. EmmaH
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    what is a restriction site?

    • one year ago
  3. Frostbite
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    lol?

    • one year ago
  4. EmmaH
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    whats so funny?

    • one year ago
  5. tammy16
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    like where i found it??

    • one year ago
  6. Frostbite
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    Well tammy said it quite clear. never the less i know you so good now that i guess you want it simplifyed? ^^

    • one year ago
  7. EmmaH
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    haha of course:)

    • one year ago
  8. EmmaH
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    and no i do not have a low IQ i just like taking things in their simplist forms

    • one year ago
  9. Frostbite
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    EmmaH one thing: That thought never orcure to me, the only reason i can this is becuase i ones was in your positon and asked for answers - So i don't judge. anyway back to the case.

    • one year ago
  10. EmmaH
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    thanks:)

    • one year ago
  11. Frostbite
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    See attachment: A restriction map tell you where on DNA string where a specific restriction enzyme is going to work: A analogy for this would be a real map: imagen you have a lot of cities with specific names (they are enzymes) and the whole map discribe how the cities are placed acording to each other.

    • one year ago
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  12. Frostbite
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    The restriction map i used as example is the plasmid pGLO

    • one year ago
  13. Frostbite
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    Remember that plasmids are DNA circles that is why it is a circle on the figure in the attachment.

    • one year ago
  14. EmmaH
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    ok i get the fact that a restriction map shows where ba restriction enzyme is but..what is a restriction enzyme?

    • one year ago
  15. Frostbite
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    A restriction enzyme is a enzyme that is able to cut the DNA into smaller strings every time there is a specfic sequence of bases.

    • one year ago
  16. EmmaH
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    oh ok so the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?

    • one year ago
  17. Frostbite
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    The most common thing is that it is a palindromic sequence

    • one year ago
  18. Frostbite
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    yea

    • one year ago
  19. EmmaH
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    palindromic sequenence?

    • one year ago
  20. Frostbite
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    Emm... palindromic comes from the Greek word palindromos and means 'coming again, which again goes the same way'. (symmetric)

    • one year ago
  21. EmmaH
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    alright so i got it earlier when i said.....the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?

    • one year ago
  22. Frostbite
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    Well: more to say that the restiction map show where restiction enzyme works on the DNA... kinda like a blueprint

    • one year ago
  23. Frostbite
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    Else you are right.

    • one year ago
  24. EmmaH
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    ooooooh ok thanks! got it

    • one year ago
  25. Frostbite
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    Last thing: this is just to show the concept of a palindromic sequenence (see attachment)

    • one year ago
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  26. EmmaH
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    thanks:)

    • one year ago
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