anonymous
  • anonymous
What is a restriction map?
Biology
chestercat
  • chestercat
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anonymous
  • anonymous
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes
anonymous
  • anonymous
what is a restriction site?
Frostbite
  • Frostbite
lol?

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anonymous
  • anonymous
whats so funny?
anonymous
  • anonymous
like where i found it??
Frostbite
  • Frostbite
Well tammy said it quite clear. never the less i know you so good now that i guess you want it simplifyed? ^^
anonymous
  • anonymous
haha of course:)
anonymous
  • anonymous
and no i do not have a low IQ i just like taking things in their simplist forms
Frostbite
  • Frostbite
EmmaH one thing: That thought never orcure to me, the only reason i can this is becuase i ones was in your positon and asked for answers - So i don't judge. anyway back to the case.
anonymous
  • anonymous
thanks:)
Frostbite
  • Frostbite
See attachment: A restriction map tell you where on DNA string where a specific restriction enzyme is going to work: A analogy for this would be a real map: imagen you have a lot of cities with specific names (they are enzymes) and the whole map discribe how the cities are placed acording to each other.
1 Attachment
Frostbite
  • Frostbite
The restriction map i used as example is the plasmid pGLO
Frostbite
  • Frostbite
Remember that plasmids are DNA circles that is why it is a circle on the figure in the attachment.
anonymous
  • anonymous
ok i get the fact that a restriction map shows where ba restriction enzyme is but..what is a restriction enzyme?
Frostbite
  • Frostbite
A restriction enzyme is a enzyme that is able to cut the DNA into smaller strings every time there is a specfic sequence of bases.
anonymous
  • anonymous
oh ok so the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?
Frostbite
  • Frostbite
The most common thing is that it is a palindromic sequence
Frostbite
  • Frostbite
yea
anonymous
  • anonymous
palindromic sequenence?
Frostbite
  • Frostbite
Emm... palindromic comes from the Greek word palindromos and means 'coming again, which again goes the same way'. (symmetric)
anonymous
  • anonymous
alright so i got it earlier when i said.....the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?
Frostbite
  • Frostbite
Well: more to say that the restiction map show where restiction enzyme works on the DNA... kinda like a blueprint
Frostbite
  • Frostbite
Else you are right.
anonymous
  • anonymous
ooooooh ok thanks! got it
Frostbite
  • Frostbite
Last thing: this is just to show the concept of a palindromic sequenence (see attachment)
1 Attachment
anonymous
  • anonymous
thanks:)

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