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EmmaH Group Title

What is a restriction map?

  • 2 years ago
  • 2 years ago

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  1. tammy16 Group Title
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    A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes

    • 2 years ago
  2. EmmaH Group Title
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    what is a restriction site?

    • 2 years ago
  3. Frostbite Group Title
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    lol?

    • 2 years ago
  4. EmmaH Group Title
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    whats so funny?

    • 2 years ago
  5. tammy16 Group Title
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    like where i found it??

    • 2 years ago
  6. Frostbite Group Title
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    Well tammy said it quite clear. never the less i know you so good now that i guess you want it simplifyed? ^^

    • 2 years ago
  7. EmmaH Group Title
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    haha of course:)

    • 2 years ago
  8. EmmaH Group Title
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    and no i do not have a low IQ i just like taking things in their simplist forms

    • 2 years ago
  9. Frostbite Group Title
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    EmmaH one thing: That thought never orcure to me, the only reason i can this is becuase i ones was in your positon and asked for answers - So i don't judge. anyway back to the case.

    • 2 years ago
  10. EmmaH Group Title
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    thanks:)

    • 2 years ago
  11. Frostbite Group Title
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    See attachment: A restriction map tell you where on DNA string where a specific restriction enzyme is going to work: A analogy for this would be a real map: imagen you have a lot of cities with specific names (they are enzymes) and the whole map discribe how the cities are placed acording to each other.

    • 2 years ago
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  12. Frostbite Group Title
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    The restriction map i used as example is the plasmid pGLO

    • 2 years ago
  13. Frostbite Group Title
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    Remember that plasmids are DNA circles that is why it is a circle on the figure in the attachment.

    • 2 years ago
  14. EmmaH Group Title
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    ok i get the fact that a restriction map shows where ba restriction enzyme is but..what is a restriction enzyme?

    • 2 years ago
  15. Frostbite Group Title
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    A restriction enzyme is a enzyme that is able to cut the DNA into smaller strings every time there is a specfic sequence of bases.

    • 2 years ago
  16. EmmaH Group Title
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    oh ok so the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?

    • 2 years ago
  17. Frostbite Group Title
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    The most common thing is that it is a palindromic sequence

    • 2 years ago
  18. Frostbite Group Title
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    yea

    • 2 years ago
  19. EmmaH Group Title
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    palindromic sequenence?

    • 2 years ago
  20. Frostbite Group Title
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    Emm... palindromic comes from the Greek word palindromos and means 'coming again, which again goes the same way'. (symmetric)

    • 2 years ago
  21. EmmaH Group Title
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    alright so i got it earlier when i said.....the restricted enzymes are like the cutting toold for when you want to splice dna and the restiction nmap nshows where restiction dna is right?

    • 2 years ago
  22. Frostbite Group Title
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    Well: more to say that the restiction map show where restiction enzyme works on the DNA... kinda like a blueprint

    • 2 years ago
  23. Frostbite Group Title
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    Else you are right.

    • 2 years ago
  24. EmmaH Group Title
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    ooooooh ok thanks! got it

    • 2 years ago
  25. Frostbite Group Title
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    Last thing: this is just to show the concept of a palindromic sequenence (see attachment)

    • 2 years ago
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  26. EmmaH Group Title
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    thanks:)

    • 2 years ago
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