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which of the following would require the use of recombinant dna

Biology
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What are the choices
crossing two apple trees to create better apples breeding a donkey and a horse to make a mule engineering bacteria that produce human insulin creating a polyploid banana tree
C

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Other answers:

Engineering bacteria to produce insulin is a use of recombinant DNA (SaveahThomas's answer), I think is correct. Crossing two apples to make better ones requires no DNA manipulation, just pollenation. Breeding a horse and a donkey does not alter the DNA sequence of either animal, and most plants are naturally polyploid.
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