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artisunder

  • 3 years ago

What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?

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  1. Reaper534
    • 3 years ago
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    hvent learnd it :(

  2. karatechopper
    • 3 years ago
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    Sorry, I haven't learnt this either.

  3. Frostbite
    • 3 years ago
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    Hmmm what kind of gel? almost guessing agarose?

  4. Frostbite
    • 3 years ago
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    If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by: \[f=6 \pi \eta r\] Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.

  5. Frostbite
    • 3 years ago
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    But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.

  6. artisunder
    • 3 years ago
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    oh wow, thank you! thank you so much!!!!

  7. Frostbite
    • 3 years ago
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    and standard plate btw. ;)

  8. ontour
    • 3 years ago
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    @Frostbite dude continue helping her :D please! shes in ap bio XD

  9. Frostbite
    • 3 years ago
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    Not sure I follow?

  10. ontour
    • 3 years ago
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    Oh. She's Advanced Placement Biology and she needs help haha.

  11. artisunder
    • 3 years ago
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    I'm in ap bio and i don't get this lab report at all haha

  12. Frostbite
    • 3 years ago
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    Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)

  13. ontour
    • 3 years ago
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    ARTI ASK THE NEXT QUESTION CHILD

  14. artisunder
    • 3 years ago
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    @Frostbite could you help me with some other questions? :)

  15. Frostbite
    • 3 years ago
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    Maybe, I'll give it a shot.

  16. artisunder
    • 3 years ago
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    you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker

  17. artisunder
    • 3 years ago
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    when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?

  18. Frostbite
    • 3 years ago
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    Hmmm, not quite sure about this sorry.

  19. artisunder
    • 3 years ago
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    oh, okay thank you though! how about this one ----> if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?

  20. Frostbite
    • 3 years ago
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    Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.

  21. Frostbite
    • 3 years ago
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    Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.

  22. artisunder
    • 3 years ago
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    i was thinking that you could rin it at half the voltage (50 v)

  23. Frostbite
    • 3 years ago
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    Oh, then i misunderstod well depends if voltage and time is porportional ;)

  24. artisunder
    • 3 years ago
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    haha i don't really know. I'm just going to out that down and hope for the best!

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