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artisunder
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What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?
 one year ago
 one year ago
artisunder Group Title
What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?
 one year ago
 one year ago

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Reaper534 Group TitleBest ResponseYou've already chosen the best response.0
hvent learnd it :(
 one year ago

karatechopper Group TitleBest ResponseYou've already chosen the best response.1
Sorry, I haven't learnt this either.
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Hmmm what kind of gel? almost guessing agarose?
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by: \[f=6 \pi \eta r\] Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
oh wow, thank you! thank you so much!!!!
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
and standard plate btw. ;)
 one year ago

ontour Group TitleBest ResponseYou've already chosen the best response.0
@Frostbite dude continue helping her :D please! shes in ap bio XD
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Not sure I follow?
 one year ago

ontour Group TitleBest ResponseYou've already chosen the best response.0
Oh. She's Advanced Placement Biology and she needs help haha.
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
I'm in ap bio and i don't get this lab report at all haha
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)
 one year ago

ontour Group TitleBest ResponseYou've already chosen the best response.0
ARTI ASK THE NEXT QUESTION CHILD
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
@Frostbite could you help me with some other questions? :)
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Maybe, I'll give it a shot.
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Hmmm, not quite sure about this sorry.
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
oh, okay thank you though! how about this one > if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
i was thinking that you could rin it at half the voltage (50 v)
 one year ago

Frostbite Group TitleBest ResponseYou've already chosen the best response.2
Oh, then i misunderstod well depends if voltage and time is porportional ;)
 one year ago

artisunder Group TitleBest ResponseYou've already chosen the best response.0
haha i don't really know. I'm just going to out that down and hope for the best!
 one year ago
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