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hvent learnd it :(
Sorry, I haven't learnt this either.
Hmmm what kind of gel? almost guessing agarose?
If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by: \[f=6 \pi \eta r\] Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.
But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.
oh wow, thank you! thank you so much!!!!
and standard plate btw. ;)
@Frostbite dude continue helping her :D please! shes in ap bio XD
Not sure I follow?
Oh. She's Advanced Placement Biology and she needs help haha.
I'm in ap bio and i don't get this lab report at all haha
Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)
ARTI ASK THE NEXT QUESTION CHILD
@Frostbite could you help me with some other questions? :)
Maybe, I'll give it a shot.
you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker
when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?
Hmmm, not quite sure about this sorry.
oh, okay thank you though! how about this one ----> if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?
Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.
Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.
i was thinking that you could rin it at half the voltage (50 v)
Oh, then i misunderstod well depends if voltage and time is porportional ;)
haha i don't really know. I'm just going to out that down and hope for the best!