anonymous
  • anonymous
What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?
Biology
  • Stacey Warren - Expert brainly.com
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SOLVED
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schrodinger
  • schrodinger
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anonymous
  • anonymous
hvent learnd it :(
karatechopper
  • karatechopper
Sorry, I haven't learnt this either.
Frostbite
  • Frostbite
Hmmm what kind of gel? almost guessing agarose?

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Frostbite
  • Frostbite
If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by: \[f=6 \pi \eta r\] Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.
Frostbite
  • Frostbite
But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.
anonymous
  • anonymous
oh wow, thank you! thank you so much!!!!
Frostbite
  • Frostbite
and standard plate btw. ;)
anonymous
  • anonymous
@Frostbite dude continue helping her :D please! shes in ap bio XD
Frostbite
  • Frostbite
Not sure I follow?
anonymous
  • anonymous
Oh. She's Advanced Placement Biology and she needs help haha.
anonymous
  • anonymous
I'm in ap bio and i don't get this lab report at all haha
Frostbite
  • Frostbite
Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)
anonymous
  • anonymous
ARTI ASK THE NEXT QUESTION CHILD
anonymous
  • anonymous
@Frostbite could you help me with some other questions? :)
Frostbite
  • Frostbite
Maybe, I'll give it a shot.
anonymous
  • anonymous
you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker
anonymous
  • anonymous
when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?
Frostbite
  • Frostbite
Hmmm, not quite sure about this sorry.
anonymous
  • anonymous
oh, okay thank you though! how about this one ----> if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?
Frostbite
  • Frostbite
Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.
Frostbite
  • Frostbite
Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.
anonymous
  • anonymous
i was thinking that you could rin it at half the voltage (50 v)
Frostbite
  • Frostbite
Oh, then i misunderstod well depends if voltage and time is porportional ;)
anonymous
  • anonymous
haha i don't really know. I'm just going to out that down and hope for the best!

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