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 2 years ago
What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?
 2 years ago
What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp?

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karatechopper
 2 years ago
Best ResponseYou've already chosen the best response.1Sorry, I haven't learnt this either.

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Hmmm what kind of gel? almost guessing agarose?

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by: \[f=6 \pi \eta r\] Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0oh wow, thank you! thank you so much!!!!

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2and standard plate btw. ;)

ontour
 2 years ago
Best ResponseYou've already chosen the best response.0@Frostbite dude continue helping her :D please! shes in ap bio XD

ontour
 2 years ago
Best ResponseYou've already chosen the best response.0Oh. She's Advanced Placement Biology and she needs help haha.

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0I'm in ap bio and i don't get this lab report at all haha

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)

ontour
 2 years ago
Best ResponseYou've already chosen the best response.0ARTI ASK THE NEXT QUESTION CHILD

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0@Frostbite could you help me with some other questions? :)

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Maybe, I'll give it a shot.

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Hmmm, not quite sure about this sorry.

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0oh, okay thank you though! how about this one > if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0i was thinking that you could rin it at half the voltage (50 v)

Frostbite
 2 years ago
Best ResponseYou've already chosen the best response.2Oh, then i misunderstod well depends if voltage and time is porportional ;)

artisunder
 2 years ago
Best ResponseYou've already chosen the best response.0haha i don't really know. I'm just going to out that down and hope for the best!
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