## artisunder Group Title What percentage gel should be used if you want to separate DNA fragments that are 25,000 bp , 21,000 bp, 10,000 bp and 6,000 bp? one year ago one year ago

1. Reaper534 Group Title

hvent learnd it :(

2. karatechopper Group Title

Sorry, I haven't learnt this either.

3. Frostbite Group Title

Hmmm what kind of gel? almost guessing agarose?

4. Frostbite Group Title

If we don't have this factor it can be hard to know becuase the driving force of the molecule or DNA is given by: $f=6 \pi \eta r$ Here f is the driving force, η is the viscosity of the medium and r is the radius of a sphere.

5. Frostbite Group Title

But if using a agarose gel I would say about 0,5 % agarose, that should cover 1k to 30k.

6. artisunder Group Title

oh wow, thank you! thank you so much!!!!

7. Frostbite Group Title

and standard plate btw. ;)

8. ontour Group Title

@Frostbite dude continue helping her :D please! shes in ap bio XD

9. Frostbite Group Title

Not sure I follow?

10. ontour Group Title

Oh. She's Advanced Placement Biology and she needs help haha.

11. artisunder Group Title

I'm in ap bio and i don't get this lab report at all haha

12. Frostbite Group Title

Well this is college bio, so I wonder if you don't have any tables to look in, I only found it, becuase I had a old table from a prof of mine. The eqautions however is stright on ;)

13. ontour Group Title

ARTI ASK THE NEXT QUESTION CHILD

14. artisunder Group Title

@Frostbite could you help me with some other questions? :)

15. Frostbite Group Title

Maybe, I'll give it a shot.

16. artisunder Group Title

you perform a restiction digest that should yield 1400 bp, 1080 bp, and 400 bp fragments. You check the restiction digest by running it on a gel that is 1.5 %, the correct percentage. but you're in a rush and only partially run the gel you also forget to load the marker

17. artisunder Group Title

when you stain the gel you only see three bands in the lane you loaded. The second band from the well is more intense than thr others and looks like a doublet. which 2 fragements make up the double? why? what could you do to further separate the fragemnts to verify this?

18. Frostbite Group Title

19. artisunder Group Title

oh, okay thank you though! how about this one ----> if you want to run gel electrophoresis at 100 v for two hours instead of one (which is how long it should take) what should you do?

20. Frostbite Group Title

Hmmm an increase in running time will allow the bands to separate even more... wich you ofcuase need to take into account if you make a aproximation. Beside from that, I most again say I'm not sure.

21. Frostbite Group Title

Might also need to adjust some equations if you have some formular that have bps as a function of length of bands travel.

22. artisunder Group Title

i was thinking that you could rin it at half the voltage (50 v)

23. Frostbite Group Title

Oh, then i misunderstod well depends if voltage and time is porportional ;)

24. artisunder Group Title

haha i don't really know. I'm just going to out that down and hope for the best!