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Anyone who would like to elaborate the nuclear Overhauser effect known from NMR? Don't fully understand why it is explained as a "through space interaction" Sorry it is a bit "off topic" from biology, but sense I work with biological molecules I choosed to post it here.

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I know it is when a nucleus affect another nucleus by irradiation as a result of dipole-dipole interactions. Which again might be a result of the spin-spin relaxation (unsure about that detale). My problem is... If was I was asked to say this in another way, I would not be able to... Which only make me think I don't understand it.

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I believe it has to do with the fact that spin polarization of one group can influence the spin of another group over time. I can't give you the exact processes that take place but it's very logical if you think about it.. Eventually everything returns to a state of equilibrium. The Nuclear Overhauser effect is the name of this process when it comes to spin polarization. At least, that's how I understood it, I'm not completely sure it's right though..
NOE is used for large biomolecules. For small ones you can guess the structure from through bonds coupling. For e.g. proteins you need to get distances between non-bonded atoms that get close in space when the protein folds. NOE gives you that. The document provided by Koikkara explains why.

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