anonymous
  • anonymous
You have completed preparing your buffer and you are ready to go home, but your supervisor informs you just before you leave that you should also prepare 0.5L of 1M Tris-base, 0.5mM EDTA, pH 7.4. The molecular weights are: Tris-base - 121.14, and EDTA -292.25. The pH of this solution will be basic. How will you pH to 7.4?
Biology
At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.

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anonymous
  • anonymous
You have completed preparing your buffer and you are ready to go home, but your supervisor informs you just before you leave that you should also prepare 0.5L of 1M Tris-base, 0.5mM EDTA, pH 7.4. The molecular weights are: Tris-base - 121.14, and EDTA -292.25. The pH of this solution will be basic. How will you pH to 7.4?
Biology
katieb
  • katieb
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At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.

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