• anonymous
Pb(OAc)2 Test: Adding Naoh and crystals of Pb(OAc)2 to fish extract, then heated will produce: What color changes? What amino acid is detected?
  • Stacey Warren - Expert
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At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.
  • chestercat
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  • Cuanchi
Reduced sulfur test: Principle: Proteins containing sulfur (in cysteine and cystine) give a black deposit of lead sulfide (PbS) when heated with lead acetate in alkaline medium.
  • Cuanchi
Procedure & observation: − To 1 ml of protein solution in a test tube, add 2 drops of 10% sodium hydroxide solution and 2 drops of lead acetate. − Mix well and put in a boiling water bath for few minutes; a black deposit is formed with albumin, while a slight black turbidity is obtained with casein due to its lower content of sulfur. Gelatin gives negative result.

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