When is the oil immersion objective used? How is the OIO used?
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Oil immersion is a technique used to increase the resolution of a microscope. This can be achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
Oil immersion objectives are used only at very large magnifications that require high resolving power.
Stained smears of mixed bacteria are recommended for this practice.
To use an oil immersion lens, first focus on the area of specimen to be observed with the high dry (400x) lens.
Place a drop of immersion oil on the cover slip over that area, and very carefully swing the oil immersion lens into place. Focus carefully, preferably by observing the lens itself while bringing it as close to the cover slip as possible, then focusing by moving the lens away from the specimen
When in focus the lens nearly touches the cover slip. The focal plane is so narrow that it is very easy to focus right past it. If you are focusing toward the specimen, you can drive the lens right into it.
this is how its used..:)
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