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JoannaBlackwelder

  • one year ago

A biochemist wishes to study the activity of an enzyme that produces one mole hydrogen ion for every mole of product produced however the enzyme is sensitive to changes in pH and will become inactive at pH less than 7.1 If the initial pH of the solution is 7.21 set with a phosphate buffer (the pKa's of the three acid-conjugate base pairs derived from phosphoric acid) H3po4 are 2.12, 7.21, and 12.32 and the reaction is designed to produce 0.15 moles of product in a volume of 250 mL what is the minimum concentration of the conjugate acid in the buffer solution?

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  1. JoannaBlackwelder
    • one year ago
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    @taramgrant0543664 @abb0t

  2. abb0t
    • one year ago
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    Perhaps the henderson-hasselbach equation might be a good choice to use here.

  3. JoannaBlackwelder
    • one year ago
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    |dw:1440641824518:dw| Like that?

  4. JoannaBlackwelder
    • one year ago
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    But wouldn't that be looking for the conjugate base, not the conjugate acid?

  5. JoannaBlackwelder
    • one year ago
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    @abb0t ?

  6. JoannaBlackwelder
    • one year ago
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    Or do I need to add the concentration of the phosphoric acid to the acid concentration?

  7. abb0t
    • one year ago
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    Since you're adding acid, I would say so yes. I'm actually not too sure, but that's what I'm thinking to do. I could get back to this later if no one has helped you yet. But I'm sure @nincompoop could help.

  8. Rushwr
    • one year ago
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    But pKa u have used is not 7.21 right? 7.21 is the initial pH right? I think we have to find pKa using the values we are given with !

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