JoannaBlackwelder
  • JoannaBlackwelder
A biochemist wishes to study the activity of an enzyme that produces one mole hydrogen ion for every mole of product produced however the enzyme is sensitive to changes in pH and will become inactive at pH less than 7.1 If the initial pH of the solution is 7.21 set with a phosphate buffer (the pKa's of the three acid-conjugate base pairs derived from phosphoric acid) H3po4 are 2.12, 7.21, and 12.32 and the reaction is designed to produce 0.15 moles of product in a volume of 250 mL what is the minimum concentration of the conjugate acid in the buffer solution?
Chemistry
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JoannaBlackwelder
  • JoannaBlackwelder
A biochemist wishes to study the activity of an enzyme that produces one mole hydrogen ion for every mole of product produced however the enzyme is sensitive to changes in pH and will become inactive at pH less than 7.1 If the initial pH of the solution is 7.21 set with a phosphate buffer (the pKa's of the three acid-conjugate base pairs derived from phosphoric acid) H3po4 are 2.12, 7.21, and 12.32 and the reaction is designed to produce 0.15 moles of product in a volume of 250 mL what is the minimum concentration of the conjugate acid in the buffer solution?
Chemistry
jamiebookeater
  • jamiebookeater
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At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.

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JoannaBlackwelder
  • JoannaBlackwelder
abb0t
  • abb0t
Perhaps the henderson-hasselbach equation might be a good choice to use here.
JoannaBlackwelder
  • JoannaBlackwelder
|dw:1440641824518:dw| Like that?

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JoannaBlackwelder
  • JoannaBlackwelder
But wouldn't that be looking for the conjugate base, not the conjugate acid?
JoannaBlackwelder
  • JoannaBlackwelder
JoannaBlackwelder
  • JoannaBlackwelder
Or do I need to add the concentration of the phosphoric acid to the acid concentration?
abb0t
  • abb0t
Since you're adding acid, I would say so yes. I'm actually not too sure, but that's what I'm thinking to do. I could get back to this later if no one has helped you yet. But I'm sure @nincompoop could help.
Rushwr
  • Rushwr
But pKa u have used is not 7.21 right? 7.21 is the initial pH right? I think we have to find pKa using the values we are given with !

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