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JoannaBlackwelder
 one year ago
A biochemist wishes to study the activity of an enzyme that produces one mole hydrogen ion for every mole of product produced however the enzyme is sensitive to changes in pH and will become inactive at pH less than 7.1 If the initial pH of the solution is 7.21 set with a phosphate buffer (the pKa's of the three acidconjugate base pairs derived from phosphoric acid) H3po4 are 2.12, 7.21, and 12.32
and the reaction is designed to produce 0.15 moles of product in a volume of 250 mL what is the minimum concentration of the conjugate acid in the buffer solution?
JoannaBlackwelder
 one year ago
A biochemist wishes to study the activity of an enzyme that produces one mole hydrogen ion for every mole of product produced however the enzyme is sensitive to changes in pH and will become inactive at pH less than 7.1 If the initial pH of the solution is 7.21 set with a phosphate buffer (the pKa's of the three acidconjugate base pairs derived from phosphoric acid) H3po4 are 2.12, 7.21, and 12.32 and the reaction is designed to produce 0.15 moles of product in a volume of 250 mL what is the minimum concentration of the conjugate acid in the buffer solution?

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JoannaBlackwelder
 one year ago
Best ResponseYou've already chosen the best response.0@taramgrant0543664 @abb0t

abb0t
 one year ago
Best ResponseYou've already chosen the best response.0Perhaps the hendersonhasselbach equation might be a good choice to use here.

JoannaBlackwelder
 one year ago
Best ResponseYou've already chosen the best response.0dw:1440641824518:dw Like that?

JoannaBlackwelder
 one year ago
Best ResponseYou've already chosen the best response.0But wouldn't that be looking for the conjugate base, not the conjugate acid?

JoannaBlackwelder
 one year ago
Best ResponseYou've already chosen the best response.0Or do I need to add the concentration of the phosphoric acid to the acid concentration?

abb0t
 one year ago
Best ResponseYou've already chosen the best response.0Since you're adding acid, I would say so yes. I'm actually not too sure, but that's what I'm thinking to do. I could get back to this later if no one has helped you yet. But I'm sure @nincompoop could help.

Rushwr
 one year ago
Best ResponseYou've already chosen the best response.0But pKa u have used is not 7.21 right? 7.21 is the initial pH right? I think we have to find pKa using the values we are given with !
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