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caozeyuan

  • one year ago

If you received an mystery transposon on a plasmid, what sort of “marker” could you insert into this transposon to determine if it is a DNA transposon or a retrotransposon

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  1. RamiroCruzo
    • one year ago
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    DNA Transposons, which move within a genome by means of a DNA intermediate Retrotransposons, which move by means of an RNA intermediate In Retrotransposition, Reverse Transcriptase makes copy of DNA by Retrotransposon RNA. So if we induce Reverse-transcriptase inhibitors (RTIs) in the plasmid sample & it doesn't show transposition its Retrotransposons otherwise its DNA Transposons

  2. Rushwr
    • one year ago
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    Transposons are segments of DNA that can move around to different positions in the genome of a single cell. There are two distinct types: Class II transposons. These consist of DNA that moves directly from place to place. Class I transposons. These are retrotransposons that first transcribe the DNA into RNA and then use reverse transcriptase to make a DNA copy of the RNA to insert in a new location.

  3. Rushwr
    • one year ago
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    I agree with u @RamiroCruzo

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