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caozeyuan
 one year ago
What effect will the following factors have on a liquid chromatographic separation? (a) column not clamped in a perfectly vertical position; (b) very slow flow rate of the mobile phase; (c) collection of large elution fractions.
caozeyuan
 one year ago
What effect will the following factors have on a liquid chromatographic separation? (a) column not clamped in a perfectly vertical position; (b) very slow flow rate of the mobile phase; (c) collection of large elution fractions.

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aaronq
 one year ago
Best ResponseYou've already chosen the best response.1so for a), because the flow is caused by gravity alone, the tilt on the column will cause the sample to unevenly interact with the stationary phase, the largest effect will be on the longitudinal diffusion and bad separation overall.dw:1443047221529:dw

aaronq
 one year ago
Best ResponseYou've already chosen the best response.1b) will allow good equilibration between phases but large band broadening  you'll have a massive, dilute band to collect instead of a tight, concentrated band.dw:1443047385789:dw

aaronq
 one year ago
Best ResponseYou've already chosen the best response.1I'm entirely sure what they mean on c) but i can speculate that the large fractions collected will contain impurities, as well as dilute concentrations of the analyte of intererest  though this shouldn't be a problem if you can evaporate the solvent used but this is not always possible.

caozeyuan
 one year ago
Best ResponseYou've already chosen the best response.0Fantastic! Thank you so much!

aaronq
 one year ago
Best ResponseYou've already chosen the best response.1for a) you will probably saturate the stationary phase leading to problems in the equilibration  i.e. mass transfer effects
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