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anonymous
 one year ago
Help!
what is a suitable experiment to determine the order of reaction between amylase and hydrogen peroxide?
Thanks a bunch! This has been puzzling me for days :S
anonymous
 one year ago
Help! what is a suitable experiment to determine the order of reaction between amylase and hydrogen peroxide? Thanks a bunch! This has been puzzling me for days :S

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Photon336
 one year ago
Best ResponseYou've already chosen the best response.2You would need to keep the concentration of one of your substrates constant, then do the same with the other to figure out the reaction rate

anonymous
 one year ago
Best ResponseYou've already chosen the best response.0I'm afraid that i still dont understand...

aaronq
 one year ago
Best ResponseYou've already chosen the best response.3You need to react these at different concentrations, (at least) 3 separate times at (at least) 2 different concentrations, and measure the rates of the reaction each time. [amylase] [hydrogen peroxide] rate Trial 1 [A] [C] 1 Trial 2 [A] [D] 2 Trial 3 [B] [C] 2 You'd then use Trials 1 and 2 (when [amylase] is constant) to find the contribution of hydrogen peroxide to the rate, that information can be used to figure out the order (exponent) for hydrogen peroxide. Similarly, you use Trials 1 and 3 (when [hydrogen peroxide] is constant) to figure out the order of amylase.

anonymous
 one year ago
Best ResponseYou've already chosen the best response.0alright thanks a bunch but, just a few things. 1) In order to determine the order of reaction, is it compulsory that you find out the order of reaction for hydrogen peroxide first? 2) Does the actual experiment have anything to do with 'the disappearing cross" ? 3) In the experiment, where is the amylase coming from? I've seen many procedures involving the enzyme catalase only and not the amylase :/ so the part where i have to obtain a procedure is a little bit tough at the moment x_x

aaronq
 one year ago
Best ResponseYou've already chosen the best response.3yeah, the enzymes don't match up. hydrogen peroxide breakdown is not catalyzed by amylase, but catalase  as you mentioned  and amylase cleaves glycosidic bonds.

anonymous
 one year ago
Best ResponseYou've already chosen the best response.0I guess I'll have to talk to my teacher then because amylase links up to the hydrolysis of starch and catalase to the decomposition of hydrogen peroxide. Anyways, thanks for your help. Much appreciated!
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