A community for students.

Here's the question you clicked on:

55 members online
  • 0 replying
  • 0 viewing

anonymous

  • one year ago

Help! what is a suitable experiment to determine the order of reaction between amylase and hydrogen peroxide? Thanks a bunch! This has been puzzling me for days :S

  • This Question is Closed
  1. Photon336
    • one year ago
    Best Response
    You've already chosen the best response.
    Medals 2

    You would need to keep the concentration of one of your substrates constant, then do the same with the other to figure out the reaction rate

  2. anonymous
    • one year ago
    Best Response
    You've already chosen the best response.
    Medals 0

    I'm afraid that i still dont understand...

  3. aaronq
    • one year ago
    Best Response
    You've already chosen the best response.
    Medals 3

    You need to react these at different concentrations, (at least) 3 separate times at (at least) 2 different concentrations, and measure the rates of the reaction each time. [amylase] [hydrogen peroxide] rate Trial 1 [A] [C] 1 Trial 2 [A] [D] 2 Trial 3 [B] [C] 2 You'd then use Trials 1 and 2 (when [amylase] is constant) to find the contribution of hydrogen peroxide to the rate, that information can be used to figure out the order (exponent) for hydrogen peroxide. Similarly, you use Trials 1 and 3 (when [hydrogen peroxide] is constant) to figure out the order of amylase.

  4. anonymous
    • one year ago
    Best Response
    You've already chosen the best response.
    Medals 0

    alright thanks a bunch but, just a few things. 1) In order to determine the order of reaction, is it compulsory that you find out the order of reaction for hydrogen peroxide first? 2) Does the actual experiment have anything to do with 'the disappearing cross" ? 3) In the experiment, where is the amylase coming from? I've seen many procedures involving the enzyme catalase only and not the amylase :/ so the part where i have to obtain a procedure is a little bit tough at the moment x_x

  5. aaronq
    • one year ago
    Best Response
    You've already chosen the best response.
    Medals 3

    yeah, the enzymes don't match up. hydrogen peroxide breakdown is not catalyzed by amylase, but catalase - as you mentioned - and amylase cleaves glycosidic bonds.

  6. anonymous
    • one year ago
    Best Response
    You've already chosen the best response.
    Medals 0

    I guess I'll have to talk to my teacher then because amylase links up to the hydrolysis of starch and catalase to the decomposition of hydrogen peroxide. Anyways, thanks for your help. Much appreciated!

  7. Not the answer you are looking for?
    Search for more explanations.

    • Attachments:

Ask your own question

Sign Up
Find more explanations on OpenStudy
Privacy Policy

Your question is ready. Sign up for free to start getting answers.

spraguer (Moderator)
5 → View Detailed Profile

is replying to Can someone tell me what button the professor is hitting...

23

  • Teamwork 19 Teammate
  • Problem Solving 19 Hero
  • You have blocked this person.
  • ✔ You're a fan Checking fan status...

Thanks for being so helpful in mathematics. If you are getting quality help, make sure you spread the word about OpenStudy.

This is the testimonial you wrote.
You haven't written a testimonial for Owlfred.