How is it possible to sort different lengths of DNA strands using gel electrophoresis?
Stacey Warren - Expert brainly.com
Hey! We 've verified this expert answer for you, click below to unlock the details :)
At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga.
Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus.
Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.
I got my questions answered at brainly.com in under 10 minutes. Go to brainly.com now for free help!
Yes it is.
The gel matrix used (generally 10% agarose) creates tortuous (zig-zaggy) paths which the DNA strands have to navigate through. The gel is subjected to an electric field and due to the negative charges on the phosphate groups (on the backbone) the strands will travel from the negative end to the positive end. The advantage is that the amount of negative charge on the strands is the same regardless of length (a strand of 10 nucleotides will have 10 negative charges and one with 100 nucleotides will have 100 negative charges) so the electric force pulling them towards the positive end is the same.
Longer ones navigate slower (because they get trapped/tangled or experience friction) while slower ones travel faster.
Uhm, can you like dumb that down? I still don't understan
Yes, smaller strands move faster, larger ones move slower across the gel.
Not the answer you are looking for? Search for more explanations.
i think, it is like... DNA electrophoresis is a method used to sort DNA molecules by length. Pieces of DNA are suspended in a tray of gel and subjected to an electric field, which causes them to migrate toward one end of the tray. The DNA separates out into bands, with the distance from the electrode corresponding to length of the strand. The technique plays a role in identifying genes for diagnosing disease and for other forms of genetic research. @jennithemeani